TY - JOUR
T1 - Honeybee royal jelly and nobiletin stimulate CRE-mediated transcription in ERK-independent and -dependent fashions, respectively, in PC12D cells
AU - Fujiwara, Hironori
AU - Kogure, Ako
AU - Sakamoto, Masahiro
AU - Yamakuni, Tohru
AU - Mimaki, Yoshihiro
AU - Murata, Kiyoshi
AU - Hitomi, Nobuyuki
AU - Yamaguchi, Kikuji
AU - Ohizumi, Yasushi
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription.
AB - To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription.
KW - Alzheimer's disease
KW - CRE-mediated transcription
KW - Honeybee royal jelly
UR - http://www.scopus.com/inward/record.url?scp=84860412128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860412128&partnerID=8YFLogxK
U2 - 10.1254/jphs.11001SC
DO - 10.1254/jphs.11001SC
M3 - Article
C2 - 21747212
AN - SCOPUS:84860412128
VL - 116
SP - 384
EP - 387
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
SN - 1347-8648
IS - 4
ER -