Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band Ip36

Ying Zhang Chen, Eiichi Soeda, Hong Wei Yang, Junko Takita, Ling Chai, Akira Horii, Johji Inazawa, Misao Ohki, Yasuhide Hayashi

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12 Citations (Scopus)

Abstract

Recent molecular studies have shown a relatively high rate of loss of heterozygosity (LOH) in neuroblastoma (NB) as well as other types of tumors in human chromosome band Ip36. To identify candidate tumor suppressor genes in NB, we searched for homozygous deletions in NB cell lines with PCR according to a high-density sequence tagged site (STS)-content map spanning Ip35-36. Among 25 NB cell lines examined, only one cell line, NB-I, showed no signal with 27 STSs in a 480 kb region in Ip36.2. The sequence analysis has revealed that the defective region included seven known genes (E4, KIFIB, SCYA5, PGD, Cortistatin, DFF45, and PEX14), nine expressed sequence tags (ESTs), and two microsatellite markers. These genes are related to apoptosis, an ubiquitin-proteasome pathway, a neuronal microtubule-associated motor molecule, and components of a common translocation machinery. The region between the DFF45 and KIFIB genes was defined as homozygous deletion by Southern blotting. The search in LOH regions with high-density STSs may be useful for the isolation and identification of tumor suppressor genes in other tumors as well as NBs.

Original languageEnglish
Pages (from-to)326-332
Number of pages7
JournalGenes Chromosomes and Cancer
Volume31
Issue number4
DOIs
Publication statusPublished - 2001 Jul 19

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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    Chen, Y. Z., Soeda, E., Yang, H. W., Takita, J., Chai, L., Horii, A., Inazawa, J., Ohki, M., & Hayashi, Y. (2001). Homozygous deletion in a neuroblastoma cell line defined by a high-density STS map spanning human chromosome band Ip36. Genes Chromosomes and Cancer, 31(4), 326-332. https://doi.org/10.1002/gcc.1151