Abstract
A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.
Original language | English |
---|---|
Pages (from-to) | 349-355 |
Number of pages | 7 |
Journal | Gene |
Volume | 36 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1985 |
Keywords
- (Recombinant DNA
- computer-aided comparison
- nucleotide sequence
- open reading frame
- photolyase)
- plasmid vector
- ultraviolet light
- yeast
ASJC Scopus subject areas
- Genetics