Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli

Akira Yasui; Simon A. Langeveld

doi:10.1016/0378-1119(85)90190-8

Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli

Akira Yasui, Simon A. Langeveld

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.

Original language	English
Pages (from-to)	349-355
Number of pages	7
Journal	Gene
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(85)90190-8
Publication status	Published - 1985

Keywords

(Recombinant DNA
computer-aided comparison
nucleotide sequence
open reading frame
photolyase)
plasmid vector
ultraviolet light
yeast

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(85)90190-8

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title = "Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli",

abstract = "A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.",

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T1 - Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli

AU - Yasui, Akira

AU - Langeveld, Simon A.

PY - 1985

Y1 - 1985

N2 - A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.

AB - A cloned fragment of Saccharomyces cerevisiae chromosomal DNA carrying the photoreactivation gene (PHR) has been sequenced. The fragment contains a 1695-bp intronless open reading frame (ORF) coding for a polypeptide of 564 amino acids (aa). The phr gene of Escherichia coli was also sequenced, and the sequence is in agreement with the published data. The yeast PHR gene has a G + C content of 36.2%, whereas 53.7% was found for the E. coli gene. Despite the difference in G + C content there is a 35% homology between the deduced aa sequences. This homology suggests that both genes have originated from a common ancestral gene.

KW - (Recombinant DNA

KW - computer-aided comparison

KW - nucleotide sequence

KW - open reading frame

KW - photolyase)

KW - plasmid vector

KW - ultraviolet light

KW - yeast

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JO - Gene

JF - Gene

SN - 0378-1119

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Homology between the photoreactivation genes of Saccharomyces cerevisiae and Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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