Histamine H1 receptor-mediated inhibition of potassium-evoked release of 5-hydroxytryptamine from mouse forebrains

Li Zi Son, Kazuhiko Yanai, Jalal Izadi Mobarakeh, Atsuro Kuramasu, Zheng Yan Li, Eiko Sakurai, Yasuhiko Hashimoto, Takeshi Watanabe, Takehiko Watanabe

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


The release of endogenous serotonin and dopamine from slices of mouse forebrains induced by high extracellular K+ was examined in histamine H1 receptor knockout mice. The release of 5-hydroxytryptamine (5-HT) evoked by 30 mM K+ significantly decreased in the presence of 10-50 μM histamine in wild-type mice, but was not inhibited in the mutant mice. Histamine H1 receptor-mediated inhibition of serotonin release in wild-type mice was also observed in the presence of thioperamide, an H3 antagonist. From these data, we postulate that endogenous histamine indirectly inhibits the release of 5-HT through H1 receptors in addition to H3 receptors. The treatment of 2 μM tetrodotoxin could partly abolish the effects of histamine on K+-evoked 5-HT release. Bicuculline, a GABAA antagonist, could reverse the histamine-induced inhibition of 5-HT release in wild-type mice, suggesting that H1 receptors facilitate the release of GABA, which in turn inhibits 5-HT release through GABAA receptors. The difference in the effects of d- and l-chlorpheniramine on K+-evoked 5-HT release in wild-type mice further supports the evidence of the function of H1 receptor modulating 5-HT release.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalBehavioural Brain Research
Issue number2
Publication statusPublished - 2001 Oct 15


  • 5-Hydroxytryptamine release
  • Antidepressants
  • Anxiety
  • Brain slice
  • Chlorpheniramine
  • Histamine
  • Histamine H1 receptor

ASJC Scopus subject areas

  • Behavioral Neuroscience


Dive into the research topics of 'Histamine H1 receptor-mediated inhibition of potassium-evoked release of 5-hydroxytryptamine from mouse forebrains'. Together they form a unique fingerprint.

Cite this