Highly-sensitive simultaneous detection of lanthanide(III) ions as kinetically stable aromatic polyaminocarboxylato complexes via capillary electrophoresis using resolution enhancement with carbonate ion

Shingo Saito, Hitoshi Hoshino

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

An aromatic polyaminocarboxylate ligand, 1-(4-aminobenzyl)ethylenediamine- N,N,N′,N′-tetraacetate (ABEDTA), is proposed as a complexing reagent in the pre-capillary mode so as to form kinetically inert Ln(III) complexes, meaning that no added ligand is necessary in alkaline carrier buffer solutions. In addition, highly-sensitive detection is possible through a light-absorbing moiety of an aminobenzyl group in the ligand. The fine-tuning of the electrophoretic mobilities of the Ln-abedta complexes is successfully achieved by adding an auxiliary carbonate ion ligand which alters the charge-to-size ratio of the complexes through fast exchange equilibria in a carrier buffer. In fact, all of the complexes are detectable with very similar analytical sensitivity and acceptable resolution (except for Ln=Sm, Eu, Gd) by using NaOH-borate carrier buffer solution at pH 12.35 with 20 mM of Na 2CO3. A typical detection limit for Tb(III) ion (to 3σ) is as low as 0.94 μM, which translates to an absolute amount of 9.4 fmol in a 1.0×10-8 dm-3 (10 nL) injection.

Original languageEnglish
Pages (from-to)1644-1647
Number of pages4
JournalAnalytical and Bioanalytical Chemistry
Volume378
Issue number6
DOIs
Publication statusPublished - 2004 Mar 1

Keywords

  • Capillary electrophoresis
  • Inert complex
  • Lanthanide ions
  • Polyaminocarboxylate
  • Ternary complex

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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