Highly sensitive fluorescence microscope system for single biological cell using laser excitation and digital image processing and display

Shun ichi Sato, Hiromasa Ito, Humio Inaba, Yoshio Taguchi, Morio Kasai

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A novel type of laser-excited fluorescence microscope system is reported, which, for the first time, realizes excellent sensitivity and spatial resolution in biological and medical applications using an ultra-high sensitivity image camera and digital image processing technique for three-dimensional display of the detected fluorescence intensity across a single cell. Experimental studies are also presented on the fluorescence intensity distribution of Rhodamine 6G adsorbed in an OH gel as a simple cell model and also of a hematoporphyrin-derivative (HpD) taken up by a cultured esophagus cancer cell.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalOptical and Quantum Electronics
Volume18
Issue number1
DOIs
Publication statusPublished - 1986 Jan 1

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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