Highly induced DNA recombination mediated by membrane permeabilized recombinant cre protein in mouse primary cells

Shizu Hidema, Takamitsu Maruyama, Shigeki Kato, Katsuhiko Nishimori

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.

    Original languageEnglish
    Pages (from-to)817-820
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume71
    Issue number3
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Cre recombinase
    • Membrane transduction
    • Transactivator of transcription-protein transduction domain (TAT-PTD)

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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