Highly induced DNA recombination mediated by membrane permeabilized recombinant cre protein in mouse primary cells

Shizu Hidema, Takamitsu Maruyama, Shigeki Kato, Katsuhiko Nishimori

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

To establish efficient induction of Cre mediated DNA recombination in primary cells, mouse embryonic fibroblast, keratinocyte, and primary preosteoblast, we tested various recombinant Cres by fusing of protein transduction domain in human immunodeficiency virus (HIV) transactivator of transcription (TAT-PTD) to the N- and/or C-terminus. HTC, modified Cre with PTD at the N-terminus, achieved the highest activity of DNA recombination for those primary cells.

Original languageEnglish
Pages (from-to)817-820
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number3
DOIs
Publication statusPublished - 2007 Mar 30

Keywords

  • Cre recombinase
  • Membrane transduction
  • Transactivator of transcription-protein transduction domain (TAT-PTD)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Highly induced DNA recombination mediated by membrane permeabilized recombinant cre protein in mouse primary cells'. Together they form a unique fingerprint.

Cite this