Higher Sensitivity in Induction of Apoptosis in Fibroblast Cell Lines Derived from LEC Strain Rats to UV-Irradiation

Masanobu Hayashi, Kazuhisa Uehara, Yukako Ichikawa, Soichiro Arai, Emiko Isogai, Toyo Okui

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The proportion of S-phase cells in a WKAH rat cell population decreased and that of G1-phase cells increased at 8 and 18 hr post-incubation following UV-irradiation, although no significant change was observed in the ratio of the proportion of S-phase to G1-phase cells in a LEC rat cell population. Thus, UV-radiation-induced delay in the progression from the G1 to S phase was observed in WKAH rat cells but was not apparent in LEC rat cells. The fraction of LEC rat cells containing a sub-GO DNA content increased with post-incubation time after UV-irradiation, but not that of WKAH rat cells. The proportion of the sub-GO fraction in LEC rat cells increased with increasing doses of UV-rays. Low molecular weight DNAs extracted from UV-irradiated LEC rat cells exhibited an intense DNA ladder pattern at 18 and 24 hr post-irradiation by electrophoretic analysis, but not those from UV-irradiated WKAH rat cells. These results showed a higher sensitivity of LEC rat cells in induction of apoptosis than that of WKAH rat cells to UV-irradiation, although there was no difference in the survival curves among the cell lines from LEC and WKAH rats after UV-irradiation.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalJournal of Veterinary Medical Science
Volume60
Issue number2
DOIs
Publication statusPublished - 1998 Feb

Keywords

  • Apoptosis
  • Fibroblast
  • LEC strain rat
  • UV-irradiation

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Higher Sensitivity in Induction of Apoptosis in Fibroblast Cell Lines Derived from LEC Strain Rats to UV-Irradiation'. Together they form a unique fingerprint.

Cite this