High wall shear stress gradient suppress morphological responses of endothelial cells to fluid flow

M. Sato, N. Saito, N. Sakamoto, T. Ohashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Arterial bifurcations are known as the common sites for development of cerebral aneurysms. Although localization of aneurysms suggests that high wall shear stress (WSS) and high wall shear stress gradient (WSSG) occurring at the bifurcations may be crucial factors for endothelial dysfunction involved in aneurysm formation, the details of the relationship between the hemodynamic environment and endothelial cell (EC) responses still remain unclear. In this study, we observed morphological responses of ECs under high WSS and high WSSG condition using a T-shaped flow chamber and under high WSS without WSSG condition using a parallel plate flow chamber to evaluate the effects of WSSG to ECs, After 24 h exposure to flow, ECs under high WSS (10 Pa) without WSSG condition oriented perpendicular to the flow, whereas ECs at high WSS (10 Pa) with WSSG condition did not cause EC alignment. After 72 h exposure to flow, ECs exposed to WSSG were not polarized whereas ECs at high WSS without WSSG condition orientated and elongated to the direction of flow. These results indicate that a WSSG may suppress orientation of ECs to the flow direction.

Original languageEnglish
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Subtitle of host publicationImage Processing, Biosignal Processing, Modelling and Simulation, Biomechanics
Pages312-313
Number of pages2
Edition4
DOIs
Publication statusPublished - 2009 Dec 1
EventWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics - Munich, Germany
Duration: 2009 Sep 72009 Sep 12

Publication series

NameIFMBE Proceedings
Number4
Volume25
ISSN (Print)1680-0737

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics
CountryGermany
CityMunich
Period09/9/709/9/12

Keywords

  • Cell orientation
  • Endothelial cell
  • Morphology
  • Wall shear stress
  • Wall shear stress gradient

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Sato, M., Saito, N., Sakamoto, N., & Ohashi, T. (2009). High wall shear stress gradient suppress morphological responses of endothelial cells to fluid flow. In World Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics (4 ed., pp. 312-313). (IFMBE Proceedings; Vol. 25, No. 4). https://doi.org/10.1007/978-3-642-03882-2-82