High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates

Toshimi Hasegawa, Manabu Hayashi, Kazuki Nomura, Mayumi Hayashi, Miyuki Kido, Tsuneo Ohmori, Masao Fukuda, Akinori Iguchi, Shigeaki Ueno, Toru Shigematsu, Masao Hirayama, Tomoyuki Fujii

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Using microplates as pressure and cultivation vessels, a high-throughput method was developed for analyzing the high-pressure inactivation kinetics of microorganisms. The loss of viability from a high-pressure treatment, measured based on the growth delay during microplate cultivation, showed reproducibility with the conventional agar plate method and was applicable for the kinetics analysis.

Original languageEnglish
Pages (from-to)788-791
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number6
DOIs
Publication statusPublished - 2012 Jun

Keywords

  • Escherichia coli
  • High-pressure
  • High-throughput
  • Microbial inactivation
  • Microplate
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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