High-throughput fluorescence polarization measurement system towards molecular interaction analysis

Osamu Wakao, Ken Sumiyoshi, Chikaaki Mizokuchi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This report demonstrated simultaneous fluorescence polarization (FP) measurement for 100 samples. The measurement was based on the synchronization detection between the switching of a liquid crystal (LC) layer and the sampling rate of an image sensor. This offered captured FP signals from multisample as single FP image, and the values of FP signals were obtained separately by image analysis. The results showed the analytical performance of our system was comparable to that of conventional apparatus and which implies our system can be applicable to molecular interaction analysis.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages557-558
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • FPIA
  • Fluorescence Polarization
  • Molecular Interaction Analysis

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Wakao, O., Sumiyoshi, K., Mizokuchi, C., Maeki, M., Ishida, A., Tani, H., Shigemura, K., Hibara, A., & Tokeshi, M. (2020). High-throughput fluorescence polarization measurement system towards molecular interaction analysis. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 557-558). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.