High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development

Makoto Endo, Tohru Tsuchiya, Kazuki Hamada, Shingo Kawamura, Kentaro Yano, Masahiro Ohshima, Atsushi Higashitani, Masao Watanabe, Makiko Kawagishi-Kobayashi

Research output: Contribution to journalArticlepeer-review

143 Citations (Scopus)

Abstract

Plant male reproductive development is highly organized and sensitive to various environmental stressors, including high temperature. We have established an experimental procedure to evaluate high temperature injury in japonica rice plants. High temperature treatment (39°C/30°C) starting at the microspore stage repeatedly reduced spikelet fertility in our system. Morphological observations revealed that pollen viability in plants exposed to high temperatures was lower than that in control plants. Most pollen grains in high temperature-treated plants displayed a normal round shape and stained reddish purple with Alexander's reagent; however, the pollen grains were very poorly attached and displayed limited germination on the stigma. To investigate gene regulatory mechanisms in the anther in high temperature environments, DNA microarray analysis was performed by comparing non-treated samples with samples treated with 24 d of high heat. Genes responsive to high temperatures were identified from clustering of microarray data. Among these, at least 13 were designated as high temperature-repressed genes in the anther. Expression analyses revealed that these genes were expressed specifically in the immature anther mainly in the tapetum at the microspore stage and down-regulated after 1 d of high temperature. The expression levels of Osc6, OsRAFTIN and TDR, which are tapetum-specific genes, were unaffected by high temperatures. These results suggest that not all tapetal genes are inhibited by increased temperatures and the tapetum itself is not degraded in such an environment. However, high temperatures may disrupt some of the tapetum functions required for pollen adhesion and germination on the stigma.

Original languageEnglish
Pages (from-to)1911-1922
Number of pages12
JournalPlant and Cell Physiology
Volume50
Issue number11
DOIs
Publication statusPublished - 2009 Nov

Keywords

  • High temperature stress
  • Male sterility
  • Microarray
  • Rice
  • Tapetum

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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