High-temperature-induced transposition of insertion elements in Burkholderia multivorans ATCC 17616

Yoshiyuki Ohtsubo, Hiroyuki Genka, Harunobu Komatsu, Yuji Nagata, Masataka Tsuda

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

An efficient and quantitative method to analyze the transposition of various insertion sequence (IS) elements in Burkholderia multivorans ATCC 17616 was devised. pGEN500, a plasmid carrying a Bacillus subtilis-derived sacB gene, was introduced into ATCC 17616 cells, and 25% of their sucrose-resistant derivatives were found to carry various IS elements on pGEN500. A PCR-based experimental protocol, in which a mixture of several specific primer pairs was used, revealed that pGEN500 captured, in addition to five previously reported IS elements (IS401, IS402, IS406, IS407, and IS408), three novel IS elements, ISBmu1, ISBmu2, and ISBmu3. The global transposition frequency of these IS elements was enhanced more than sevenfold under a high-temperature condition (42°C) but not under oxidative stress or starvation conditions. To our knowledge, this is the first report demonstrating the elevated transposition activities of several IS elements at a high temperature. The efficient experimental protocol developed in this study will be useful in quantitatively and simultaneously investigating various IS elements, as well as in capturing novel functional mobile elements from a wide variety of bacteria.

Original languageEnglish
Pages (from-to)1822-1828
Number of pages7
JournalApplied and environmental microbiology
Volume71
Issue number4
DOIs
Publication statusPublished - 2005 Apr

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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