TY - GEN
T1 - High-speed focusing of cells using depth-from-diffraction method
AU - Oku, Hiromasa
AU - Theodorus,
AU - Hashimoto, Koichi
AU - Ishikawa, Masatoshi
PY - 2006/12/27
Y1 - 2006/12/27
N2 - This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named "depth from diffraction", based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.
AB - This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named "depth from diffraction", based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=33845653258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845653258&partnerID=8YFLogxK
U2 - 10.1109/ROBOT.2006.1642257
DO - 10.1109/ROBOT.2006.1642257
M3 - Conference contribution
AN - SCOPUS:33845653258
SN - 0780395069
SN - 9780780395060
T3 - Proceedings - IEEE International Conference on Robotics and Automation
SP - 3636
EP - 3641
BT - Proceedings 2006 IEEE International Conference on Robotics and Automation, ICRA 2006
T2 - 2006 IEEE International Conference on Robotics and Automation, ICRA 2006
Y2 - 15 May 2006 through 19 May 2006
ER -