High-speed focusing of cells using depth-from-diffraction method

Hiromasa Oku; Theodorus; Koichi Hashimoto; Masatoshi Ishikawa

doi:10.1109/ROBOT.2006.1642257

High-speed focusing of cells using depth-from-diffraction method

Hiromasa Oku, Theodorus, Koichi Hashimoto, Masatoshi Ishikawa

INF - System Information Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named "depth from diffraction", based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.

Original language	English
Title of host publication	Proceedings 2006 IEEE International Conference on Robotics and Automation, ICRA 2006
Pages	3636-3641
Number of pages	6
DOIs	https://doi.org/10.1109/ROBOT.2006.1642257
Publication status	Published - 2006 Dec 27
Event	2006 IEEE International Conference on Robotics and Automation, ICRA 2006 - Orlando, FL, United States Duration: 2006 May 15 → 2006 May 19

Publication series

Name	Proceedings - IEEE International Conference on Robotics and Automation
Volume	2006
ISSN (Print)	1050-4729

Other

Other	2006 IEEE International Conference on Robotics and Automation, ICRA 2006
Country/Territory	United States
City	Orlando, FL
Period	06/5/15 → 06/5/19

ASJC Scopus subject areas

Software
Control and Systems Engineering
Artificial Intelligence
Electrical and Electronic Engineering

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10.1109/ROBOT.2006.1642257

Cite this

High-speed focusing of cells using depth-from-diffraction method. / Oku, Hiromasa; Theodorus, ; Hashimoto, Koichi et al.

Proceedings 2006 IEEE International Conference on Robotics and Automation, ICRA 2006. 2006. p. 3636-3641 1642257 (Proceedings - IEEE International Conference on Robotics and Automation; Vol. 2006).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Oku, H, Theodorus, , Hashimoto, K & Ishikawa, M 2006, High-speed focusing of cells using depth-from-diffraction method. in Proceedings 2006 IEEE International Conference on Robotics and Automation, ICRA 2006., 1642257, Proceedings - IEEE International Conference on Robotics and Automation, vol. 2006, pp. 3636-3641, 2006 IEEE International Conference on Robotics and Automation, ICRA 2006, Orlando, FL, United States, 06/5/15. https://doi.org/10.1109/ROBOT.2006.1642257

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title = "High-speed focusing of cells using depth-from-diffraction method",

abstract = "This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named {"}depth from diffraction{"}, based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.",

author = "Hiromasa Oku and Theodorus and Koichi Hashimoto and Masatoshi Ishikawa",

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language = "English",

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series = "Proceedings - IEEE International Conference on Robotics and Automation",

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T1 - High-speed focusing of cells using depth-from-diffraction method

AU - Oku, Hiromasa

AU - Theodorus,

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AU - Ishikawa, Masatoshi

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Y1 - 2006/12/27

N2 - This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named "depth from diffraction", based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.

AB - This paper proposes a new autofocusing method for microbiological specimens, such as cells. The proposed focusing method used a quick focus estimation named "depth from diffraction", based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the proposed method, continuous focusing was applied to focus tracking of a swimming paramecium, in combination with two-dimensional tracking. This allowed three-dimensional tracking of the paramecium for 75 s to be successfully demonstrated.

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High-speed focusing of cells using depth-from-diffraction method

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