High-speed CCD imaging system for monitoring neural activity in vivo and in vitro, using a voltage-sensitive dye

Ichiro Takashima, Michinori Ichikawa, Toshio Iijima

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    We have designed and constructed a high-speed CCD imaging system for optically detecting neural activity from preparations stained externally with a voltage-sensitive dye, and have used this system to image evoked and epileptiform neural activity in the rat somatosensory cortex. The imaging system uses a commercially available 1/3-in. CCD chip, and it can continuously capture images for more than 8 s, at 1000 frames/s, with a spatial resolution of 128 x 62 pixels. The spatial/temporal resolution of the CCD sensor is variable by changing the geometry of on-chip binning pixels, which can be controlled by a PC/AT computer. Dye bleaching correction was not necessary for long-term imaging of epileptiform neural events, since the sensitivity of the CCD sensor was increased by combining the signal from adjacent pixels.

    Original languageEnglish
    Pages (from-to)147-159
    Number of pages13
    JournalJournal of Neuroscience Methods
    Volume91
    Issue number1-2
    DOIs
    Publication statusPublished - 1999 Sep 15

    Keywords

    • CCD
    • Epileptiform discharge
    • Optical imaging
    • Somatosensory cortex
    • Voltage-sensitive dye

    ASJC Scopus subject areas

    • Neuroscience(all)

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