High-speed CCD imaging system for monitoring neural activity

I. Takashima, R. Kajiwara, T. Iijima

    Research output: Contribution to journalArticle

    Abstract

    We have designed and constructed a high-speed CCD imaging system for optically detecting neural activity from preparations stained externally with a voltage-sensitive dye, and have used this system to image evoked and epileptiform neural activity in the rat somatosensory cortex. The imaging system uses a commercially available 1/3-inch CCD chip, and it can continuously capture images for more than 8 seconds, at 1000 frames/sec, with spatial resolution of 128 × 62 pixels. The spatial/temporal resolution of the CCD sensor is variable by changing the geometry of on-chip binning pixels, which can be controlled by a PC/AT computer. Dye bleaching correction was not necessary for long-term imaging of epileptiform neural events, since the sensitivity of the CCD sensor was increased by combining the signal from adjacent pixels.

    Original languageEnglish
    Pages (from-to)11-25
    Number of pages15
    JournalDenshi Gijutsu Sogo Kenkyusho Iho/Bulletin of the Electrotechnical Laboratory
    Volume63
    Issue number8-9
    Publication statusPublished - 1999 Dec 1

    ASJC Scopus subject areas

    • Condensed Matter Physics
    • Electrical and Electronic Engineering

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