Abstract
We have designed and constructed a high-speed CCD imaging system for optically detecting neural activity from preparations stained externally with a voltage-sensitive dye, and have used this system to image evoked and epileptiform neural activity in the rat somatosensory cortex. The imaging system uses a commercially available 1/3-inch CCD chip, and it can continuously capture images for more than 8 seconds, at 1000 frames/sec, with spatial resolution of 128 × 62 pixels. The spatial/temporal resolution of the CCD sensor is variable by changing the geometry of on-chip binning pixels, which can be controlled by a PC/AT computer. Dye bleaching correction was not necessary for long-term imaging of epileptiform neural events, since the sensitivity of the CCD sensor was increased by combining the signal from adjacent pixels.
Original language | English |
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Pages (from-to) | 11-25 |
Number of pages | 15 |
Journal | Denshi Gijutsu Sogo Kenkyusho Iho/Bulletin of the Electrotechnical Laboratory |
Volume | 63 |
Issue number | 8-9 |
Publication status | Published - 1999 Dec 1 |
ASJC Scopus subject areas
- Condensed Matter Physics
- Electrical and Electronic Engineering