High-speed autofocusing of a cell using diffraction patterns

Hiromasa Oku, Masatoshi Ishikawa, Theodorus, Koichi Hashimoto

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

This paper proposes a new autofocusing method for observing cells under a transmission illumination. The focusing method uses a quick and simple focus estimation technique termed "depth from diffraction," which is based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed autofocusing. To demonstrate the method, it was applied to continuous focus tracking of a swimming paramecium, in combination with two-dimensional position tracking. Three-dimensional tracking of the Paramecium for 70 s was successfully demonstrated.

Original languageEnglish
Pages (from-to)3952-3960
Number of pages9
JournalOptics Express
Volume14
Issue number9
DOIs
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'High-speed autofocusing of a cell using diffraction patterns'. Together they form a unique fingerprint.

Cite this