Abstract
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases. Seiriki et al. developed a very high-speed serial-sectioning imaging system named FAST that allows whole-brain imaging at a spatial resolution to image all brain cells and long-range neuronal projections in experimental animal models and facilitates animal-to-human translational research.
Original language | English |
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Pages (from-to) | 1085-1100.e6 |
Journal | Neuron |
Volume | 94 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2017 Jun 21 |
Externally published | Yes |
Keywords
- cell distribution
- human post-mortem brain
- non-human primate brain
- subcellular resolution
- whole-brain imaging
ASJC Scopus subject areas
- Neuroscience(all)