High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA

Masayuki Hayashida, Akira Yamaguchi, Hiroaki Misawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 μM.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume44
Issue number50-52
DOIs
Publication statusPublished - 2005 Dec 16

Keywords

  • Au nanopartiole
  • DNA hybridization
  • In situ
  • Label-free
  • SPR

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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