High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA

Masayuki Hayashida; Akira Yamaguchi; Hiroaki Misawa

doi:10.1143/JJAP.44.L1544

High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA

Masayuki Hayashida, Akira Yamaguchi, Hiroaki Misawa

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 μM.

Original language	English
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	44
Issue number	50-52
DOIs	https://doi.org/10.1143/JJAP.44.L1544
Publication status	Published - 2005 Dec 16

Keywords

Au nanopartiole
DNA hybridization
In situ
Label-free
SPR

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1143/JJAP.44.L1544

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title = "High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA",

abstract = "In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 μM.",

keywords = "Au nanopartiole, DNA hybridization, In situ, Label-free, SPR",

author = "Masayuki Hayashida and Akira Yamaguchi and Hiroaki Misawa",

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language = "English",

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T1 - High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA

AU - Hayashida, Masayuki

AU - Yamaguchi, Akira

AU - Misawa, Hiroaki

PY - 2005/12/16

Y1 - 2005/12/16

N2 - In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 μM.

AB - In this study, we demonstrate the use of surface plasmon interaction between Au nanoparticles (GPs) and a Au thin film for enhancement of surface plasmon resonance (SPR) spectroscopy sensing for DNA hybridization. In situ DNA hybridization with a Au-nanoparticle-attached probe DNA (DNA-GP) monolayer is monitored by SPR spectroscopy, and the results reveal that the DNA-GP allows detection of unlabeled target DNA with the large dynamic range of 1 pM to 10 μM.

KW - Au nanopartiole

KW - DNA hybridization

KW - In situ

KW - Label-free

KW - SPR

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JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

SN - 0021-4922

IS - 50-52

ER -

High sensitivity and large dynamic range surface plasmon resonance sensing for DNA hybridization using au-nanoparticle-attached probe DNA

Abstract

Keywords

ASJC Scopus subject areas

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