Abstract
Sensitive liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry (MS) can be used to analyze the bile acid composition of rat serum. This method can analyze eight common bile acids and their glycine and taurine conjugates in 100 μl rodent serum by gradient elution on a reversed-phase column using a mixture of 20 mM ammonium acetate buffer (pH 8.0), acetonitrile and methanol as a mobile phase. Selected reaction monitoring analysis under negative ion detection mode allowed the achievement of a high sensitive assay with a simple solid phase extraction using an ODS cartridge column. We used this method to investigate the effect of a one-day fast on the concentration and composition of serum bile acids in rats. The results suggested that the method described here is useful for the dynamic analysis of serum bile acids in rats.
Original language | English |
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Pages (from-to) | 1179-1186 |
Number of pages | 8 |
Journal | Journal of Pharmaceutical and Biomedical Analysis |
Volume | 40 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2006 Mar 18 |
Externally published | Yes |
Keywords
- Bile acid
- Electrospray ionization
- LC-MS/MS
- Rat serum
- Simultaneous determination
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry