High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry

Masayuki Ando; Tohru Kaneko; Ritsuko Watanabe; Satoshi Kikuchi; Takaaki Goto; Takashi Iida; Takanori Hishinuma; Nariyasu Mano; Junichi Goto

doi:10.1016/j.jpba.2005.09.013

High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry

Masayuki Ando, Tohru Kaneko, Ritsuko Watanabe, Satoshi Kikuchi, Takaaki Goto, Takashi Iida, Takanori Hishinuma, Nariyasu Mano, Junichi Goto

University Hospital (not affiliated with any section)

Research output: Contribution to journal › Article

59 Citations (Scopus)

Abstract

Sensitive liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry (MS) can be used to analyze the bile acid composition of rat serum. This method can analyze eight common bile acids and their glycine and taurine conjugates in 100 μl rodent serum by gradient elution on a reversed-phase column using a mixture of 20 mM ammonium acetate buffer (pH 8.0), acetonitrile and methanol as a mobile phase. Selected reaction monitoring analysis under negative ion detection mode allowed the achievement of a high sensitive assay with a simple solid phase extraction using an ODS cartridge column. We used this method to investigate the effect of a one-day fast on the concentration and composition of serum bile acids in rats. The results suggested that the method described here is useful for the dynamic analysis of serum bile acids in rats.

Original language	English
Pages (from-to)	1179-1186
Number of pages	8
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	40
Issue number	5
DOIs	https://doi.org/10.1016/j.jpba.2005.09.013
Publication status	Published - 2006 Mar 18

Keywords

Bile acid
Electrospray ionization
LC-MS/MS
Rat serum
Simultaneous determination

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

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Ando, M., Kaneko, T., Watanabe, R., Kikuchi, S., Goto, T., Iida, T., Hishinuma, T., Mano, N., & Goto, J. (2006). High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 40(5), 1179-1186. https://doi.org/10.1016/j.jpba.2005.09.013

High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry. / Ando, Masayuki; Kaneko, Tohru; Watanabe, Ritsuko; Kikuchi, Satoshi; Goto, Takaaki; Iida, Takashi; Hishinuma, Takanori; Mano, Nariyasu; Goto, Junichi.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 40, No. 5, 18.03.2006, p. 1179-1186.

Research output: Contribution to journal › Article

Ando, Masayuki ; Kaneko, Tohru ; Watanabe, Ritsuko ; Kikuchi, Satoshi ; Goto, Takaaki ; Iida, Takashi ; Hishinuma, Takanori ; Mano, Nariyasu ; Goto, Junichi. / High sensitive analysis of rat serum bile acids by liquid chromatography/electrospray ionization tandem mass spectrometry. In: Journal of Pharmaceutical and Biomedical Analysis. 2006 ; Vol. 40, No. 5. pp. 1179-1186.

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abstract = "Sensitive liquid chromatography (LC)/electrospray ionization (ESI) tandem mass spectrometry (MS) can be used to analyze the bile acid composition of rat serum. This method can analyze eight common bile acids and their glycine and taurine conjugates in 100 μl rodent serum by gradient elution on a reversed-phase column using a mixture of 20 mM ammonium acetate buffer (pH 8.0), acetonitrile and methanol as a mobile phase. Selected reaction monitoring analysis under negative ion detection mode allowed the achievement of a high sensitive assay with a simple solid phase extraction using an ODS cartridge column. We used this method to investigate the effect of a one-day fast on the concentration and composition of serum bile acids in rats. The results suggested that the method described here is useful for the dynamic analysis of serum bile acids in rats.",

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author = "Masayuki Ando and Tohru Kaneko and Ritsuko Watanabe and Satoshi Kikuchi and Takaaki Goto and Takashi Iida and Takanori Hishinuma and Nariyasu Mano and Junichi Goto",

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AU - Iida, Takashi

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KW - LC-MS/MS

KW - Rat serum

KW - Simultaneous determination

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