TY - JOUR
T1 - High-resolution pH imaging sensor for microscopic observation of microorganisms
AU - Nakao, Motoi
AU - Inoue, Shinji
AU - Yoshinobu, Tatsuo
AU - Iwasaki, Hiroshi
N1 - Funding Information:
This work was supportedb y the Asahi Glass Foundation and by a Grant-in-Aid for DevelopmenStc ientific Research (ContractN o. 05558113)f rom the Japanese Ministry of EducationS, cience,S portsand Culture. The authora re gratefult o Mr. R. Oishi and Mr. T. Fukushige at Nakano Vinegar Co, Ltd. for technicasl upporto n ex-perimenot f microorganisms.
PY - 1996/8
Y1 - 1996/8
N2 - We have constructed a high-resolution pH imaging sensor, which enables one to observe the metabolic activity of microorganisms microscopically. A spatial resolution better than 10 μm was achieved by thinning the Si substrate to 20 μm, and by employing an infrared laser as an illumination source. Using a 100 kHz digital sampling technique for signal detection, a pH image of 128 x 128 pixels was measured within 15 min. The pH imaging sensor was integrated into a system for microscopic observation of incubated biological specimens such as microorganisms. Using the pH imaging sensor, Escherichia coli (E. coli) colonies incubated on agar medium for 8 h (visual size ∼0.2 mm diameter, number of cells ∼800) were detected. The observed pH distribution was in agreement with the result of simulation based on the production and diffusion of acidic ions.
AB - We have constructed a high-resolution pH imaging sensor, which enables one to observe the metabolic activity of microorganisms microscopically. A spatial resolution better than 10 μm was achieved by thinning the Si substrate to 20 μm, and by employing an infrared laser as an illumination source. Using a 100 kHz digital sampling technique for signal detection, a pH image of 128 x 128 pixels was measured within 15 min. The pH imaging sensor was integrated into a system for microscopic observation of incubated biological specimens such as microorganisms. Using the pH imaging sensor, Escherichia coli (E. coli) colonies incubated on agar medium for 8 h (visual size ∼0.2 mm diameter, number of cells ∼800) were detected. The observed pH distribution was in agreement with the result of simulation based on the production and diffusion of acidic ions.
KW - Chemical imaging sensor
KW - Light-addressable potentiometric sensor
KW - pH imaging sensor
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U2 - 10.1016/S0925-4005(96)01903-X
DO - 10.1016/S0925-4005(96)01903-X
M3 - Article
AN - SCOPUS:0042826485
SN - 0925-4005
VL - 34
SP - 234
EP - 239
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1-3
ER -