The spatial resolution of the pH-imaging sensor, which enables one to observe the pH-distribution in an electrolyte, was improved up to 10μm, by thinning the Si substrate to 20μm, optimizing the wavelength of the illumination source, and employing digital sampling technique for signal detection. The pH-imaging sensor was integrated into a system for microscopic observation of microorganisms and incubated biological specimens. As an application of the pH-imaging sensor, Escherichia coli (E. coli) colonies on agar medium were observed after 8 hours of incubation (visual size approx.0.2mmφ, number of cells approx.800). The observed pH-distribution was in agreement with the simulated one based on the production and diffusion of acidic ions.
|Number of pages||4|
|Publication status||Published - 1995|
|Event||Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2) - Stockholm, Sweden|
Duration: 1995 Jun 25 → 1995 Jun 29
|Other||Proceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2)|
|Period||95/6/25 → 95/6/29|
ASJC Scopus subject areas