High-resolution pH-imaging sensor for microscopic observation of microorganisms

Motoi Nakao, Shinji Inoue, Tatsuo Yoshinobu, Hiroshi Iwasaki

Research output: Contribution to conferencePaperpeer-review

2 Citations (Scopus)

Abstract

The spatial resolution of the pH-imaging sensor, which enables one to observe the pH-distribution in an electrolyte, was improved up to 10μm, by thinning the Si substrate to 20μm, optimizing the wavelength of the illumination source, and employing digital sampling technique for signal detection. The pH-imaging sensor was integrated into a system for microscopic observation of microorganisms and incubated biological specimens. As an application of the pH-imaging sensor, Escherichia coli (E. coli) colonies on agar medium were observed after 8 hours of incubation (visual size approx.0.2mmφ, number of cells approx.800). The observed pH-distribution was in agreement with the simulated one based on the production and diffusion of acidic ions.

Original languageEnglish
Pages451-454
Number of pages4
Publication statusPublished - 1995
Externally publishedYes
EventProceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2) - Stockholm, Sweden
Duration: 1995 Jun 251995 Jun 29

Other

OtherProceedings of the 1995 8th International Conference on Solid-State Sensors and Actuators and Eurosensors IX. Part 1 (of 2)
CityStockholm, Sweden
Period95/6/2595/6/29

ASJC Scopus subject areas

  • Engineering(all)

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