TY - GEN
T1 - HIGH RESOLUTION, ONE-SHOT IMAGING OF CELLULAR ACTIVITY OF SPHEROIDS BY OXYGEN DEPENDENT ELECTROCHEMILUMINESCENCE
AU - Hiramoto, Kaoru
AU - Ino, Kosuke
AU - Komatsu, Keika
AU - Nashimoto, Yuji
AU - Shiku, Hitoshi
N1 - Funding Information:
This work was supported by Grant-in-Aid for JSPS Fellows (No.20J21401) from the Japan Society for the Promotion of Science (JSPS), and by System Design of Inclusive Society Living with Infectious Diseases (SDGS-ID), Young Researcher Support Project, Tohoku University.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We propose a facile imaging technique to visualize O2 consumption of cellular spheroids using electrochemiluminescence (ECL) of L-012, a luminol analog. The ECL was generated via electrochemical reduction of O2, so that the luminescence intensity directly corresponded to the concentration of O2, which further utilized for visualizing O2 consumption profile of spheroids. The proposed system offers high throughput imaging of cellular activity of multiple samples with comparable resolution to a conventional electrochemical method.
AB - We propose a facile imaging technique to visualize O2 consumption of cellular spheroids using electrochemiluminescence (ECL) of L-012, a luminol analog. The ECL was generated via electrochemical reduction of O2, so that the luminescence intensity directly corresponded to the concentration of O2, which further utilized for visualizing O2 consumption profile of spheroids. The proposed system offers high throughput imaging of cellular activity of multiple samples with comparable resolution to a conventional electrochemical method.
KW - Electrochemiluminescence
KW - Live cell imaging
KW - Oxygen consumption
KW - Spheroid
UR - http://www.scopus.com/inward/record.url?scp=85136990752&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136990752&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136990752
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1443
EP - 1444
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -