High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

Shihomi Masuda, Yuhki Yanase, Eiji Usukura, Sou Ryuzaki, Pangpang Wang, Koichi Okamoto, Thasaneeya Kuboki, Satoru Kidoaki, Kaoru Tamada

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.

Original languageEnglish
Article number3720
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • General

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