High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures

Juan C. Sanchez, Melissa Carrillo, Suraj Pandey, Moraima Noda, Luis Aldama, Denisse Feliz, Elin Claesson, Weixiao Yuan Wahlgren, Gregory Tracy, Phu Duong, Angela C. Nugent, Andrew Field, Vukica Šrajer, Christopher Kupitz, So Iwata, Eriko Nango, Rie Tanaka, Tomoyuki Tanaka, Luo Fangjia, Kensuke TonoShigeki Owada, Sebastian Westenhoff, Marius Schmidt, Emina A. Stojković

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phytochromes (PHYs) are photoreceptor proteins first discovered in plants, where they control a variety of photomorphogenesis events. PHYs as photochromic proteins can reversibly switch between two distinct states: a red light (Pr) and a far-red light (Pfr) absorbing form. The discovery of Bacteriophytochromes (BphPs) in nonphotosynthetic bacteria has opened new frontiers in our understanding of the mechanisms by which these natural photoswitches can control single cell development, although the role of BphPs in vivo remains largely unknown. BphPs are dimeric proteins that consist of a photosensory core module (PCM) and an enzymatic domain, often a histidine kinase. The PCM is composed of three domains (PAS, GAF, and PHY). It holds a covalently bound open-chain tetrapyrrole (biliverdin, BV) chromophore. Upon absorption of light, the double bond between BV rings C and D isomerizes and reversibly switches the protein between Pr and Pfr states. We report crystal structures of the wild-type and mutant (His275Thr) forms of the canonical BphP from the nonphotosynthetic myxobacterium Stigmatella aurantiaca (SaBphP2) in the Pr state. Structures were determined at 1.65 Å and 2.2 Å (respectively), the highest resolution of any PCM construct to date. We also report the room temperature wild-type structure of the same protein determined at 2.1 Å at the SPring-8 Angstrom Compact free electron LAser (SACLA), Japan. Our results not only highlight and confirm important amino acids near the chromophore that play a role in Pr-Pfr photoconversion but also describe the signal transduction into the PHY domain which moves across tens of angstroms after the light stimulus.

Original languageEnglish
Article number054701
JournalStructural Dynamics
Volume6
Issue number5
DOIs
Publication statusPublished - 2019 Sep 1
Externally publishedYes

ASJC Scopus subject areas

  • Radiation
  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy

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    Sanchez, J. C., Carrillo, M., Pandey, S., Noda, M., Aldama, L., Feliz, D., Claesson, E., Wahlgren, W. Y., Tracy, G., Duong, P., Nugent, A. C., Field, A., Šrajer, V., Kupitz, C., Iwata, S., Nango, E., Tanaka, R., Tanaka, T., Fangjia, L., ... Stojković, E. A. (2019). High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures. Structural Dynamics, 6(5), [054701]. https://doi.org/10.1063/1.5120527