High-performance liquid chromatography with photodiode array detection for determination of nobiletin content in the brain and serum of mice administrated the natural compound

Daisuke Saigusa, Masatoshi Shibuya, Daisuke Jinno, Hiroyuki Yamakoshi, Yoshiharu Iwabuchi, Akihito Yokosuka, Yoshihiro Mimaki, Akira Naganuma, Yasushi Ohizumi, Yoshihisa Tomioka, Tohru Yamakuni

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We recently demonstrated that nobiletin, a citrus flavonoid, exhibits anti-dementia action in animals. However, no determination methods for the content of nobiletin with beneficial action in the brain of nobiletin- administered animals have been developed, nor has its pharmacokinetics been revealed completely. Here, we established the high-performance liquid chromatography/photodiode array detection method for nobiletin determination using Bond Elut C18 SPE cartridges for extraction, where the calibration curve was linear over 0.025-10 ng, with coefficient of variation of less than 6.76%. This method enabled us to determine pharmacokinetic parameters of nobiletin given intraperitoneally or per os in the brain of mice.

Original languageEnglish
Pages (from-to)3635-3641
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume400
Issue number10
DOIs
Publication statusPublished - 2011 Jul

Keywords

  • Brain
  • Dementia
  • High-performance liquid chromatography
  • Nobiletin
  • Pharmacokinetics
  • Photodiode array

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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