High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

Koji Muramoto; Hisao Kamiya; Hiroshi Kawauchi; Hiroshi Meguro

doi:10.2116/analsci.1.447

High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi, Hiroshi Meguro

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.

Original language	English
Pages (from-to)	447-450
Number of pages	4
Journal	analytical sciences
Volume	1
Issue number	5
DOIs	https://doi.org/10.2116/analsci.1.447
Publication status	Published - 1985

Keywords

Fluorescein isothiocyanate
fluorescent reagent
high performance liquid chromatography

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/analsci.1.447

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title = "High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products",

abstract = "Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.",

keywords = "Fluorescein isothiocyanate, fluorescent reagent, high performance liquid chromatography",

author = "Koji Muramoto and Hisao Kamiya and Hiroshi Kawauchi and Hiroshi Meguro",

year = "1985",

doi = "10.2116/analsci.1.447",

language = "English",

volume = "1",

pages = "447--450",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "Japan Society for Analytical Chemistry",

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T1 - High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

AU - Muramoto, Koji

AU - Kamiya, Hisao

AU - Kawauchi, Hiroshi

AU - Meguro, Hiroshi

PY - 1985

Y1 - 1985

N2 - Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.

AB - Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.

KW - Fluorescein isothiocyanate

KW - fluorescent reagent

KW - high performance liquid chromatography

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U2 - 10.2116/analsci.1.447

DO - 10.2116/analsci.1.447

M3 - Article

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VL - 1

SP - 447

EP - 450

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 5

ER -

High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

Abstract

Keywords

ASJC Scopus subject areas

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