High Performance Liquid Chromatography of Fluorescein Isothiocyanate Isomers and Intermediate Products

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi, Hiroshi Meguro

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Fluorescein isothiocyanate (FITC) isomers and the intermediate products have been separated by reversed phase high performance liquid chromatography (HPLC), using a linear gradient formed from acetone and 0,01 M sodium phosphate buffer (pH 7.0) or an isocratic elution of 40% acetone in 0.1 M pyridine-acetic acid (pH 7,0). This HPLC system was used to evaluate commercial FITC products. A method for the purification of the FITC product by HPLC was also described.

    Original languageEnglish
    Pages (from-to)447-450
    Number of pages4
    Journalanalytical sciences
    Volume1
    Issue number5
    DOIs
    Publication statusPublished - 1985 Jan 1

    Keywords

    • Fluorescein isothiocyanate
    • fluorescent reagent
    • high performance liquid chromatography

    ASJC Scopus subject areas

    • Analytical Chemistry

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