High-Performance Liquid Chromatography of Estrogen 3-Sulfates with Electrochemical Detection Using an Immobilized Enzyme Reactor

Toshifumi Niwa, Tomoyoshi Koshiyama, Shunji Ishiwata, Junichi Goto, Toshio Nambara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple and sensitive method for the determination of estrogen 3-sulfates by high-performance liquid chromatography with electrochemical detection is described. The sulfates were separated on a reversed-phase column and subsequently hydrolyzed with an immobilized sulfatase reactor in a continuous flow mode. Estrogens liberated were then detected with a coulometric detector. A linear response was observed for four sulfates, estrone sulfate, 16 α - hydroxyestrone 3-sulfate, estradiol 3-sulfate and estriol 3-sulfate in the range 25-1000 ng as an injected amount. The detection limits (S/N = 10) were 20 ng for estriol 3-sulfate and 16 α -hydroxyestrone 3-sulfate, and 50 ng for estrone sulfate and estradiol 3-sulfate, respectively.

Original languageEnglish
Pages (from-to)242-246
Number of pages5
JournalJapanese Journal of Clinical Chemistry
Volume20
Issue number4
DOIs
Publication statusPublished - 1991 Jan 1

Keywords

  • electrochemical detection
  • estrogen
  • high-performance liquid chromatography
  • immobilized enzyme reactor
  • sulfate

ASJC Scopus subject areas

  • Clinical Biochemistry

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