The separation of 3-N-acetylglucosaminides of cholate, chenodeoxycholate, deoxycholate, ursodeoxycholate and lithocholate, and their glycine- and taurine-conjugates has been carried out by high-performance liquid chromatography on a reversed-phase column. The chromatographic behavior of bile acid 3-N-acetylglucosaminides was dependent on the number and positions of hydroxyl groups and the structure of the side-chain. The bile acid 3-N-acetylglucosaminides were efficiently separated according to their conjugated form on a Cosmosil 5C18column using a 0.3% potassium phosphate buffer-aceto-nitrile system. The chromatographic separation of ursodeoxycholate 3- and 7-N-acetylglucosaminides is also discussed.
ASJC Scopus subject areas
- Molecular Medicine