High-Performance Liquid Chromatographic Method for the Analysis of Nτ-Methylhistidine in Food, Chicken Excreta, and Rat Urine

Kunioki Hayashi, Yoshizane Maeda, Masaaki Toyomizu, Yuichiro Tomita

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and post-column fluorescence derivatization for the analysis of Nτ-methylhistidine in food, chicken excreta, and rat urine is described. Nτ-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The Nτ-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15 mM sodium octane sulfonate in 20 mM potassium phosphate), and subjected to high-performance liquid chromatography.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalJournal of nutritional science and vitaminology
Issue number2
Publication statusPublished - 1987


  • N-methylhistidine
  • high-performance liquid chromatography

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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