TY - JOUR
T1 - High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis
AU - Sobajima, J.
AU - Ozaki, S.
AU - Uesugi, H.
AU - Osakada, F.
AU - Inoue, M.
AU - Fukuda, Y.
AU - Shirakawa, H.
AU - Yoshida, M.
AU - Rokuhara, A.
AU - Imai, H.
AU - Kiyosawa, K.
AU - Nakao, K.
N1 - Funding Information:
This research was supported by grants from the National Science Foundation (BNS-82-18791 and BNS-83-19629), the Swan Fund, the Sigma X i Society, the National Institute of Mental Health Small Grants program (1-ro3-mh38300-01), and Faculty research Funds from the University of Massachusetts. The authors would l i k e t o thank Denise Zwahlen and Nadine Peacock for their field observations and their support in this project.
PY - 1999
Y1 - 1999
N2 - Background - High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti- neutrophil cytoplasmic antibodies (pANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported. Aims - To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH). Patients - Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested. Methods - ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay. Results - p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of antinuclear antibody or anti-smooth muscle antibody. Conclusions - HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.
AB - Background - High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti- neutrophil cytoplasmic antibodies (pANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported. Aims - To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH). Patients - Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested. Methods - ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay. Results - p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of antinuclear antibody or anti-smooth muscle antibody. Conclusions - HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.
KW - Autoimmune
KW - Chromosomal proteins
KW - Hepatitis
KW - High mobility group 1 and 2
KW - Perinuclear anti-neutrophil cytoplasmic antibodies
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U2 - 10.1136/gut.44.6.867
DO - 10.1136/gut.44.6.867
M3 - Article
C2 - 10323891
AN - SCOPUS:0032981786
VL - 44
SP - 867
EP - 873
JO - Gut
JF - Gut
SN - 0017-5749
IS - 6
ER -