Abstract
Phospholipase A1 (PLA1) is a hydrolytic enzyme that catalyzes the removal of the acyl group from position 1 of lecithin to form lysolecithin. The PLA1 gene, which had been cloned from Aspergillus oryzae, was expressed in Saccharomyces cerevisiae and A. oryzae. Through the modification of the medium composition and the feeding conditions of substrate, the production level of PLA1 by S. cerevisiae was increased to a level fivefold higher than that indicated in a previous report. In the case of A. oryzae, introduction of multicopies of PLA1 expression units, and the morphological change from the pellet form to the filamentous form were effective for the enhancement of PLA1 production. We succeeded in producing 3,500 U/ml of PLA1 using an industrial-scale fermentor.
Original language | English |
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Pages (from-to) | 94-101 |
Number of pages | 8 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 65 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2001 Jan |
Keywords
- Aspergillus oryzae
- CPY
- Mycelial morphology
- Phospholipase A
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry