High-level expression of viral interleukin-10 in cardiac allografts fails to prolong graft survival

Osamu Adachi, Eiji Yamato, Shunsuke Kawamoto, Mayu Yamamoto, Hideaki Tahara, Koichi Tabayashi, Jun Ichi Miyazaki

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Background. Viral interleukin (vIL)-10, encoded in the Epstein-Barr virus genome, shares many of the anti-inflammatory properties of cellular IL-10 but is supposed to lack IL-10's immunostimulatory properties. Thus, vIL-10 is expected to offer superior immunosuppression. Methods. We established transgenic mice (vIL-10 Tg) that express vIL-10 systemically and transplanted their hearts as vascularized allografts into unmodified major histocompatibility complex (MHC) full-mismatch or MHC class II-disparate mice. Results. The vIL-10 Tg mice revealed high-level expression of vIL-10 in major organs including the heart. However, the heart grafts from the vIL-10 Tg mice failed to exhibit prolonged survival in combination with either the MHC full-mismatch or the class II-disparate mice. In the MHC class II-disparate mice, the vIL-10 Tg heart grafts showed severe CD8+ T-cell infiltration and increased interferon (IFN)-γ mRNA expression compared with non-Tg grafts. Conclusion. High level expression of vIL-10 in grafts can exacerbate immunological rejection in an allogenic transplantation model.

Original languageEnglish
Pages (from-to)1603-1608
Number of pages6
JournalTransplantation
Volume74
Issue number11
DOIs
Publication statusPublished - 2002 Dec 15

ASJC Scopus subject areas

  • Transplantation

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