High-level expression of maize phosphoenolpyruvate carboxylase in transgenie rice plants

Maurice S.B. Ku, Sakae Agarie, Mika Nomura, Hiroshi Fukayama, Hiroko Tsuchida, Kazuko Ono, Sakiko Hirose, Seiichi Toki, Mitsue Miyao, Makoto Matsuoka

Research output: Contribution to journalArticle

301 Citations (Scopus)

Abstract

Using an Agrobacterium-mediated transformation system, we have introduced the intact gene of maize phosphoenolpyruvate carboxylase (PEPC), which catalyzes the initial fixation of atmospheric CO2 in C4 plants into the C3 crop rice. Most transgenic rice plants showed high-level expression of the maize gene; the activities of PEPC in leaves of some transgenic plants were two- to threefold higher than those in maize, and the enzyme accounted for up to 12% of the total leaf soluble protein. RNA gel blot and Southern blot analyses showed that the level of expression of the maize PEPC in transgenic rice plants correlated with the amount of transcript and the copy number of the inserted maize gene. Physiologically, the transgenic plants exhibited reduced O2 inhibition of photosynthesis and photosynthetic rates comparable to those of untransformed plants. The results demonstrate a successful strategy for installing the key biochemical component of the C4 pathway of photosynthesis in C3 plants.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalNature Biotechnology
Volume17
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1
Externally publishedYes

Keywords

  • C photosynthesis pathway
  • Maize
  • Phosphoenolpyruvate carboxylase
  • Rice
  • Transformation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Ku, M. S. B., Agarie, S., Nomura, M., Fukayama, H., Tsuchida, H., Ono, K., Hirose, S., Toki, S., Miyao, M., & Matsuoka, M. (1999). High-level expression of maize phosphoenolpyruvate carboxylase in transgenie rice plants. Nature Biotechnology, 17(1), 76-81. https://doi.org/10.1038/5256