High-level expression and characterization of fully active recombinant congereel galectins in eschericia coli

Tomohisa Ogawa, Chihiro Ishii, Yuji Suda, Hisao Kamiya, Koji Muramoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An expression system for recombinant conger eel galectins, congerins I and II, were constructed using the pTV 118N plasmid vector and Escherichia coli.Recombinant congerins I and II could be obtained in the soluble active form with high quantitative yield. Mutation of codons for Val andLeu located in the N-terminal region of Con I increased the expression efficiency. Purification of recombinant proteins were done by only two chromatographical steps from E. coli extract. The purified recombinant congerins were found to be almost the same as the native ones except for the acetyl group at the N-terminus; that is, they showed the same structures and carbohydrate binding activities, suggesting that N-terminal acetyl groups of congerins were not significant for activity.

Original languageEnglish
Pages (from-to)476-480
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • Codon usage
  • Conger eel
  • Escherichia coli
  • Galectin
  • Recombinant protein

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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