High frequency of Acinetobacter soli among Acinetobacter isolates causing bacteremia at a tertiary hospital in Japan

Shiro Endo, Hisakazu Yano, Hajime Kanamori, Shinya Inomata, Tetsuji Aoyagi, Masumitsu Hatta, Yoshiaki Gu, Koichi Tokuda, Miho Kitagawa, Mitsuo Kaku

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Acinetobacter baumannii is generally the most frequently isolated Acinetobacter species. Sequence analysis techniques allow reliable identification of Acinetobacter isolates at the species level. Forty-eight clinical isolates of Acinetobacter spp. were obtained from blood cultures at Tohoku University Hospital. These isolates were identified at the species level by partial sequencing of the RNA polymerase β-subunit (rpoB), 16S rRNA, and gyrB genes. Then further characterization was done by using the PCR for detection of OXA-type β-lactamase gene clusters, metallo-βlactamases, and carO genes. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing were also performed. The most frequent isolate was Acinetobacter soli (27.1%). Six of the 13 A. soli isolates were carbapenem nonsusceptible, and all of these isolates produced IMP-1. PFGE revealed that the 13 A. soli isolates were divided into 8 clusters. This study demonstrated that A. soli accounted for a high proportion of Acinetobacter isolates causing bacteremia at a Japanese tertiary hospital. Non-A. baumannii species were identified more frequently than A. baumannii and carbapenem-nonsusceptible isolates were found among the non-A. baumannii strains. These results emphasize the importance of performing epidemiological investigations of Acinetobacter species.

Original languageEnglish
Pages (from-to)911-915
Number of pages5
JournalJournal of Clinical Microbiology
Volume52
Issue number3
DOIs
Publication statusPublished - 2014 Mar

ASJC Scopus subject areas

  • Microbiology (medical)

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