High Expression of YB-1 Gene in Erythroid Cells in Patients with Refractory Anemia

Hisayuki Yokoyama, Hideo Harigae, Shinichiro Takahashi, Junichi Kameoka, Koichi Miyamura, Kenichi Ishizawa, Mitsuo Kaku, Takeshi Sasaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

It has been shown that aberrant expression of a transcription factor, GATA-1, leads to maturation arrest and transformation of erythroid cells. We previously reported that a multifunctional protein, YB-1, was expressed strongly in the spleen of a GATA-1 mutant mouse, which was filled with transformed erythroblasts. This finding suggested that YB-1 has roles in erythropoiesis. In this study, we examined in vivo expression of YB-1 messenger RNA (mRNA) in bone marrow erythroid cells and erythroid leukemic cell lines. During erythroid differentiation of erythroid leukemic cell lines, the expression level of YB-1 mRNA was highest at the early phase of differentiation and then decreased. In human bone marrow cells, the in vivo expression level of YB-1 mRNA was higher in glycophorin A-positive cells than in glycophorin A-negative cells. An interesting finding was that expression of YB-1 was higher in erythroblasts in myelodysplastic syndrome-refractory anemia (MDS-RA) than in normal cells. The findings suggested that YB-1 functions in the early stage of erythropoiesis and that aberrant expression of this protein may induce hematological diseases such as MDS.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalInternational journal of hematology
Volume78
Issue number3
DOIs
Publication statusPublished - 2003 Oct

Keywords

  • Erythropoiesis
  • GATA factors
  • Myelodysplastic syndrome
  • YB-1 gene

ASJC Scopus subject areas

  • Hematology

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