High expression of apolipoprotein E mRNA in the brains with sporadic Alzheimer's disease

Kaoru Yamagata, Katsuya Urakami, Kazuyuki Ikeda, Yong Ji, Yoshiki Adachi, Hiroyuki Arai, Hidetada Sasaki, Kenzo Sato, Kenji Nakashima

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

In order to study progressive dementia in Alzheimer's disease (AD) patients, we analyzed the gene expression of apolipoprotein E (apoE). ApoE mRNA level in the brains of patients with AD was determined by the reverse transcriptase-polymerase chain reaction (RT-PCR) method. ApoE genotype and the promoter polymorphisms (-491A/T, Th1/E47cs) were determined by the PCR restriction fragment length polymorphism method. The apoE mRNA level was significantly higher in the AD group than the control group (p!0.05). ApoE mRNA in the AD group with apoE ε4 was also significantly higher than that in the control group with apoE ε4 (p < 0.05). Our result did not indicate the possibility that the promoter polymorphisms modulate the apoE mRNA level. The higher level of apoE mRNA in AD with apoE ε4 may play an important role in the development of AD.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalDementia and geriatric cognitive disorders
Volume12
Issue number2
DOIs
Publication statusPublished - 2001

Keywords

  • Apolipoprotein E
  • Apolipoprotein E mRNA
  • Apolipoprotein Eε4
  • Sporadic Alzheimer's disease

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Cognitive Neuroscience
  • Psychiatry and Mental health

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