High affinity anti-inorganic material antibody generation by integrating graft and evolution technologies: Potential of antibodies as biointerface molecules

Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Takanari Togashi, Nozomi Yokoo, Hiroya Abe, Satoshi Ohara, Tadafumi Adschiri, Izumi Kumagai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Recent advances in molecular evolution technology enabled us to identify peptides and antibodies with affinity for inorganic materials. In the field of nanotechnology, the use of the functional peptides and antibodies should aid the construction of interface molecules designed to spontaneously link different nanomaterials; however, few material-binding antibodies, which have much higher affinity than short peptides, have been identified. Here, we generated high affinity antibodies from material-binding peptides by integrating peptide-grafting and phage-display techniques.Amaterial-binding peptide sequence was first grafted into an appropriate loop of the complementarity determining region (CDR) of a camel-type single variable antibody fragment to create a low affinity material-binding antibody. Application of a combinatorial library approach to another CDR loop in the low affinity antibody then clearly and steadily promoted affinity for a specific material surface. Thermodynamic analysis demonstrated that the enthalpy synergistic effect from grafted and selectedCDRloops drastically increased the affinity for material surface, indicating the potential of antibody scaffold for creating high affinity small interface units. We show the availability of the construction of antibodies by integrating graft and evolution technology for various inorganic materials and the potential of high affinity material-binding antibodies in biointerface applications.

Original languageEnglish
Pages (from-to)7784-7793
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number10
DOIs
Publication statusPublished - 2010 Mar 5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'High affinity anti-inorganic material antibody generation by integrating graft and evolution technologies: Potential of antibodies as biointerface molecules'. Together they form a unique fingerprint.

  • Cite this