Heterologous expression of aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast saccharomyces cerevisiae

Jitsuro Kaneda, Kenji Sasaki, Katsuya Gomi, Takahiro Shintani

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

abs The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

Original languageEnglish
Pages (from-to)168-170
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number1
DOIs
Publication statusPublished - 2011 Jan 28

Keywords

  • Aspergillus oryzae
  • Ethanol
  • Saccharomyces cerevisiae
  • Xylose fermentation
  • Xylose reductase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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