Heterologous expression of aspergillus oryzae xylose reductase and xylitol dehydrogenase genes facilitated xylose utilization in the yeast saccharomyces cerevisiae

Jitsuro Kaneda, Kenji Sasaki, Katsuya Gomi, Takahiro Shintani

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    abs The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

    Original languageEnglish
    Pages (from-to)168-170
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Volume75
    Issue number1
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Aspergillus oryzae
    • Ethanol
    • Saccharomyces cerevisiae
    • Xylose fermentation
    • Xylose reductase

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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