Heterologous biosynthesis of fungal indole sesquiterpene sespendole

Kosei Kudo, Chengwei Liu, Tomoyuki Matsumoto, Atsushi Minami, Taro Ozaki, Hiroaki Toshima, Katsuya Gomi, Hideaki Oikawa

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Indole sesquiterpene sespendole, which has been isolated from the filamentous fungus Pseudobotrytis terrestris FKA-25, is a specific inhibitor of lipid droplet synthesis in mouse macro-phages. The biosynthetic pathway that involves genes encoding six enzymes (spdEMBQHJ) was elucidated through heterologous expression of spd genes in Aspergillus oryzae, biotransfor-mation experiments, and in vitro enzymatic reactions with a re-combinant protein, thereby revealing the mechanism underlying the characteristic modification on the indole ring, catalyzed by a set of prenyltransferase (SpdE)/cytochrome P450 (SpdJ) enzymes. Functional analysis of the homologous genes encoding these enzymes involved in the biosynthesis of lolitrem allowed a biosynthetic pathway for the bicyclic ring skeleton fused to the indole ring to be proposed.

    Original languageEnglish
    Pages (from-to)1492-1497
    Number of pages6
    JournalChemBioChem
    Volume19
    Issue number14
    DOIs
    Publication statusPublished - 2018 Jul 16

    Keywords

    • Biosynthesis
    • Enzymes
    • Gene sequencing
    • Natural products
    • Terpenoids

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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