Heterogeneous fatty acid uptake early after reperfusion in rat hearts

Yuriko Yamane, Nobumasa Ishide, Yutaka Kagaya, Daiya Takeyama, Nobuyuki Shiba, Masanobu Chida, Tetsuji Nozaki, Toshihiro Takahashi, Tatsuo Ido, Kunio Shirato

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3 Citations (Scopus)

Abstract

We determined whether spatial distributions of substrate uptake are heterogeneous within the area at risk during reperfusion. Quantitative autoradiography with imaging plates and two long-lived radioisotopes was applied to 15 open-chest, anesthetized rats subjected to 30 min of coronary artery ligation and 30 min of reperfusion. Regions showing increased [β- methyl-[1-14C]heptadecanoic acid ([14C]BMHDA) uptake (166 ± 17% of that in the nonischemic area) appeared at the lateral borders and subepicardial layer within the area at risk, and 2-deoxy-D-[1-3H]glucose ([3H]DG) uptake was 103 ± 24% in these regions. Regions with decreased [14C]BMHDA uptake (28 ± 11%) occupied the midmyocardial layer except at the lateral borders within the area at risk, and [3H]DG uptake was 62 ± 18% in these regions. The percentage interregional coefficientsof variation (index of heterogeneity) in [14C]BMHDA uptake, [3H]DG uptake, and blood flow were higher in the area at risk than in the nonischemic area (76 ± 23 vs. 21 ± 7%, 39 ± 10 vs. 21 ± 7%, and 49 ± 19 vs. 14 ± 4%, respectively). Heterogeneous distributions of substrate uptake may explain the conflicting results concerning substrate metabolism during reperfusion.

Original languageEnglish
Pages (from-to)H923-H929
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume274
Issue number3 43-3
Publication statusPublished - 1998 Mar 1

Keywords

  • Double-tracer autoradiography
  • Glucose metabolism
  • Imaging plate

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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  • Cite this

    Yamane, Y., Ishide, N., Kagaya, Y., Takeyama, D., Shiba, N., Chida, M., Nozaki, T., Takahashi, T., Ido, T., & Shirato, K. (1998). Heterogeneous fatty acid uptake early after reperfusion in rat hearts. American Journal of Physiology - Heart and Circulatory Physiology, 274(3 43-3), H923-H929.