TY - JOUR
T1 - Hepatic and serum bile acid compositions in patients with biliary atresia
T2 - A microanalysis using gas chromatography-mass spectrometry with negative ion chemical ionization detection
AU - Abukawa, Daiki
AU - Nakagawa, Michiko
AU - Iinuma, Kazuie
AU - Nio, Masaki
AU - Ohi, Ryoji
AU - Goto, Junichi
PY - 1998/8
Y1 - 1998/8
N2 - Hepatic and serum bile acids in five patients with biliary atresia were preoperatively determined by microanalysis using gas chromatography-mass spectrometry with negative ion chemical ionization detection. The hepatic content of total bile acids was markedly elevated (3079 ± 711 nmol/g protein), most of which were primary bile acids. Accumulation of unconjugated bile acids (2.93% to 4.62% of the total) was observed in the liver tissue of these patients, although only trace amounts were detected in their sera. The ratio of glycine-conjugated to taurine-conjugated bile acids was 0.44±0.18 in liver tissue and 0.79±0.52 in serum and these values were significantly lower than those of controls. This study has shown that the composition of bile acids in serum does not reflect that in liver tissue faithfully. The accumulation of these hydrophobic bile acids may contribute to initiating or exacerbating liver injury in infants with cholestatic liver diseases.
AB - Hepatic and serum bile acids in five patients with biliary atresia were preoperatively determined by microanalysis using gas chromatography-mass spectrometry with negative ion chemical ionization detection. The hepatic content of total bile acids was markedly elevated (3079 ± 711 nmol/g protein), most of which were primary bile acids. Accumulation of unconjugated bile acids (2.93% to 4.62% of the total) was observed in the liver tissue of these patients, although only trace amounts were detected in their sera. The ratio of glycine-conjugated to taurine-conjugated bile acids was 0.44±0.18 in liver tissue and 0.79±0.52 in serum and these values were significantly lower than those of controls. This study has shown that the composition of bile acids in serum does not reflect that in liver tissue faithfully. The accumulation of these hydrophobic bile acids may contribute to initiating or exacerbating liver injury in infants with cholestatic liver diseases.
KW - Biliary atresia
KW - Gas chromatography-mass spectrometry
KW - Hepatic bile acids
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U2 - 10.1620/tjem.185.227
DO - 10.1620/tjem.185.227
M3 - Article
C2 - 9865469
AN - SCOPUS:0032130802
VL - 185
SP - 227
EP - 237
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 4
ER -