Heparan sulfate proteoglycan is an important attachment factor for cell entry of Akabane and Schmallenberg viruses

Shin Murakami, Akiko Takenaka-Uema, Tomoya Kobayashi, Kentaro Kato, Masayuki Shimojima, Massimo Palmarini, Taisuke Horimoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Akabane virus (AKAV) and Schmallenberg virus (SBV) are members of the genus Orthobunyavirus, which are transmitted by arthropod vectors with a broad cellular tropism in vitro as well as in vivo. Both AKAV and SBV cause arthrogryposishydranencephaly syndrome in ruminants. The main cellular receptor and attachment factor for entry of these orthobunyaviruses are unknown. Here, we found that AKAV and SBV infections were inhibited by the addition of heparin or enzymatic removal of cell surface heparan sulfates. To confirm this finding, we prepared heparan sulfate proteoglycan (HSPG)-knockout (KO) cells by using a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system and measured the quantities of binding of these viruses to cell surfaces. We observed a substantial reduction in AKAV and SBV binding to cells, limiting the infections by these viruses. These data demonstrate that HSPGs are important cellular attachment factors for AKAV and SBV, at least in vitro, to promote virus replication in susceptible cells.

Original languageEnglish
Article numbere00503-17
JournalJournal of virology
Volume91
Issue number15
DOIs
Publication statusPublished - 2017 Aug 1
Externally publishedYes

Keywords

  • Akabane virus
  • Cell entry
  • Heparan sulfate
  • Schmallenberg virus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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  • Cite this

    Murakami, S., Takenaka-Uema, A., Kobayashi, T., Kato, K., Shimojima, M., Palmarini, M., & Horimoto, T. (2017). Heparan sulfate proteoglycan is an important attachment factor for cell entry of Akabane and Schmallenberg viruses. Journal of virology, 91(15), [e00503-17]. https://doi.org/10.1128/JVI.00503-17