Hemichrome Determination by Thermal Lensing with Polyethylene Glycols for Signal Enhancement in Aqueous Solutions

Viktoriya R. Galimova, Mingqiang Liu, Mladen Franko, Dmitry S. Volkov, Akihide Hibara, Mikhail A. Proskurnin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The thermal lens technique is proposed for the determination of total hemoglobin in the form of reversible hemichrome. The conditions were optimized (concentration of sodium dodecyl sulfate, 2 mM) to attain the maximum sensitivity with the use of polyethylene glycols as signal enhancers. For polyethylene glycols with molecular weights 1500–35000 Da in a concentration range of 5–15% w/w (5–25 mM), the influence on thermal lens signal enhancement was estimated. It is shown that the use of 5% w/w polyethylene glycol 2000 provides the maximum increase in the thermal lens enhancement factor (by 40%) in comparison with unmodified aqueous solutions. The detection limit of iron(II) tris(1,10-phenanthrolinate) as a model system is 60 nM. Under these conditions, the thermal lens detection limit of hemichrome is 10 nM, which shows a 15-fold enhancement compared to spectrophotometry. Modification of the medium with polyethylene glycols decreases the limit of detection of hemichrome determination by 15% in comparison with unmodified aqueous solutions due to better reproducibility for the range of concentrations from 0.02 to 0.9 µM.

Original languageEnglish
Pages (from-to)1743-1762
Number of pages20
JournalAnalytical Letters
Volume51
Issue number11
DOIs
Publication statusPublished - 2018 Jul 24

Keywords

  • Hemoglobin
  • hemichrome
  • photothermal spectroscopy
  • polyethylene glycol
  • thermal lens spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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