Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle

Mizuki Hino; Seiichi Wada; Yuki Tajika; Yoshihiro Morimura; Nobuyuki Hamada; Tomoo Funayama; Tetsuya Sakashita; Takehiko Kakizaki; Yasuhiko Kobayashi; Hiroshi Yorifuji

doi:10.1247/csf.06038

Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle

Mizuki Hino, Seiichi Wada, Yuki Tajika, Yoshihiro Morimura, Nobuyuki Hamada, Tomoo Funayama, Tetsuya Sakashita, Takehiko Kakizaki, Yasuhiko Kobayashi, Hiroshi Yorifuji

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.

Original language	English
Pages (from-to)	51-56
Number of pages	6
Journal	Cell structure and function
Volume	32
Issue number	1
DOIs	https://doi.org/10.1247/csf.06038
Publication status	Published - 2007
Externally published	Yes

Keywords

Autophagy
Calcium ion influx
Heavy ion microbeam
Membrane aberration

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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10.1247/csf.06038

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title = "Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle",

abstract = "The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.",

keywords = "Autophagy, Calcium ion influx, Heavy ion microbeam, Membrane aberration",

author = "Mizuki Hino and Seiichi Wada and Yuki Tajika and Yoshihiro Morimura and Nobuyuki Hamada and Tomoo Funayama and Tetsuya Sakashita and Takehiko Kakizaki and Yasuhiko Kobayashi and Hiroshi Yorifuji",

year = "2007",

doi = "10.1247/csf.06038",

language = "English",

volume = "32",

pages = "51--56",

journal = "Cell Structure and Function",

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publisher = "Japan Society for Cell Biology",

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TY - JOUR

T1 - Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle

AU - Hino, Mizuki

AU - Wada, Seiichi

AU - Tajika, Yuki

AU - Morimura, Yoshihiro

AU - Hamada, Nobuyuki

AU - Funayama, Tomoo

AU - Sakashita, Tetsuya

AU - Kakizaki, Takehiko

AU - Kobayashi, Yasuhiko

AU - Yorifuji, Hiroshi

PY - 2007

Y1 - 2007

N2 - The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.

AB - The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.

KW - Autophagy

KW - Calcium ion influx

KW - Heavy ion microbeam

KW - Membrane aberration

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JO - Cell Structure and Function

JF - Cell Structure and Function

SN - 0386-7196

IS - 1

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Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle

Abstract

Keywords

ASJC Scopus subject areas

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