Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle

Mizuki Hino, Seiichi Wada, Yuki Tajika, Yoshihiro Morimura, Nobuyuki Hamada, Tomoo Funayama, Tetsuya Sakashita, Takehiko Kakizaki, Yasuhiko Kobayashi, Hiroshi Yorifuji

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalCell structure and function
Volume32
Issue number1
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Autophagy
  • Calcium ion influx
  • Heavy ion microbeam
  • Membrane aberration

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle'. Together they form a unique fingerprint.

Cite this