Heat treatment of egg white controls allergic symptoms and induces oral tolerance to ovalbumin in a murine model of food allergy

Hiroko Watanabe, Masako Toda, Haruko Sekido, Anne Wellner, Tomoyuki Fujii, Thomas Henle, Satoshi Hachimura, Haruyo Nakajima-Adachi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Scope: Heated foods often present low allergenicity, and have recently been used in specific oral immunotherapy for food allergies. However, the influence of heating on tolerogenicity of food allergens is not well elucidated. Here, we investigated biochemical, allergenic, and tolerogenic properties of heated egg white (EW) using a murine model of food allergy. Methods and results: Raw EWs were treated at 80°C for 15 min (80EW, mild heating condition), 100°C for 5 min (100EW, cooking condition), or 121°C for 40 min (121EW, retort pouch condition), and freeze-dried. A transgenic OVA23-3 mice model expressing T-cell receptor specific for ovalbumin (OVA, a major EW allergen) induced Th2 cells and IgE production, and presented intestinal inflammation when fed untreated EW diet. 80EW-fed mice presented only moderate inflammation but high Th2 responses. 100EW-fed mice did not present inflammation but induced tolerance as seen in reduced T-cell responses and IgE levels. 100EW demonstrated higher digestive stability and slower absorption in intestine, compared with untreated EW and 80EW. 121EW was strongly aggregated, was not absorbed well, and developed Th1 responses without tolerance induction. Conclusion: OVA in EW treated only under a particular heat condition (e.g. 100°C for 5 min) lost allergenicity, but possessed tolerogenicity.

Original languageEnglish
Pages (from-to)394-404
Number of pages11
JournalMolecular Nutrition and Food Research
Volume58
Issue number2
DOIs
Publication statusPublished - 2014 Feb 1

Keywords

  • Egg white
  • Food allergy
  • Heat treatment
  • Mouse model
  • Oral tolerance

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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