Heat and Chemical Stability of Blood Group-Specific Immunoglobulins in the State of Immunecomplex

Kaoru Sagisaka, Masayasu Noguchi, Masato Funayama, Yasuhiro Aoki

Research output: Contribution to journalArticlepeer-review

Abstract

Immune agglutinin or precipitin loses its reactivity. with the corresponding antigen by heating or some chemicals. A. Se group saliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum, respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol (2-ME) of various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150°C for 10 min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acquire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical labile.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
Journalthe tohoku journal of experimental medicine
Volume150
Issue number3
DOIs
Publication statusPublished - 1986

Keywords

  • blood group
  • chemical stability
  • heat and
  • immunecomplex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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