Healthy lifestyles are associated with higher levels of perforin, granulysin and granzymes A/B-expressing cells in peripheral blood lymphocytes

Qing Li, Kanehisa Morimoto, Ari Nakadai, Tianli Qu, Hiroki Matsushima, Masao Katsumata, Takako Shimizu, Hirofumi Inagaki, Yukiyo Hirata, Kimiko Hirata, Tomoyuki Kawada, Yuquan Lu, Kunio Nakayama, Alan M. Krensky

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Objective: It is well documented that natural killer (NK) cells provide host defense against tumors and viruses. We previously showed that lifestyle affects human NK and LAK activities. In order to explore the underlying mechanism, we investigated the effect of lifestyle on intracellular perforin, granulysin, and granzymes A/B in peripheral blood lymphocytes (PBL). Methods: 114 healthy male subjects, aged 20-59 years, from a large company in Osaka, Japan were selected with informed consent. The subjects were divided into groups reporting good, moderate, and poor lifestyles according to their responses on a questionnaire regarding eight health practices (cigarette smoking, alcohol consumption, sleeping hours, working hours, physical exercise, eating breakfast, balanced nutrition, and mental stress). Peripheral blood was taken, and numbers of NK, T, perforin, granulysin, and granzymes A/B-expressing cells in PBL were measured by flow cytometry. Results: Subjects with good or moderate lifestyle showed significantly higher numbers of NK, and perforin, granulysin, and granzymes A/B-expressing cells and a significantly lower number of T cells in PBL than subjects with poor lifestyle. Among the eight health practices, cigarette smoking, physical exercise, eating breakfast, and balanced nutrition significantly affect the numbers of NK, T cells, perforin, granulysin, and/or granzymes A/B-expressing cells, and alcohol consumption significantly affects the number of granzyme A-expressing cells. On the other hand, mental stress, sleeping, and working hours had no effect on those parameters. Conclusions: Taken together, these findings indicate that poor lifestyle significantly decreases the numbers of NK, perforin, granulysin, and granzymes A/B-expressing cells in PBL.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalPreventive Medicine
Volume44
Issue number2
DOIs
Publication statusPublished - 2007 Feb
Externally publishedYes

Keywords

  • Granulysin
  • Granzyme
  • Lifestyle
  • NK cell
  • Perforin
  • T cell

ASJC Scopus subject areas

  • Epidemiology
  • Public Health, Environmental and Occupational Health

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