Haplotype C of growth hormone (GH) gene in Japanese Black cattle: Structure of GH protein and a novel method for detection of the gene

Abolfazl Bahrami, Astrid Ardiyanti, Kaoru Tonosaki, Keiichi Suzuki, Nanako Tanida, Takuji Hirayama, Sang Gun Roh, Takeshi Nishio, Kazuo Katoh

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

From a series of studies on single nucleotide polymorphisms (SNPs) in the bovine growth hormone (GH) gene of Japanese Black cattle, the type-C (127Val and 172Met) that is specific for this breed has been intensively focused upon because of the economic importance for carcass traits, such as intramuscular oleic acid contents. In the present study, we intended to analyze the 3-D structure of GH of haplotype C, and developed a novel method to detect the type C gene. Three-D analysis of the type C protein showed that the amino acid residues (127Val and 172Met), which are present in the third and fourth helixes, respectively, and are important for binding with GH receptors, are shifted to deeper positions in the molecule compared with that for type A (127Leu and 172Thr), implying the alteration of binding interaction with receptors. A novel, efficient and cost-effective method (Dot-blot-SNP technique) for type C genotyping was successfully established, of which the basal method was a reported genotyping of SNPs for a large number of plants, reducing the cost to 10% or less of direct sequencing.

Original languageEnglish
Pages (from-to)802-804
Number of pages3
JournalAnimal Science Journal
Volume84
Issue number12
DOIs
Publication statusPublished - 2013 Dec

Keywords

  • 3-D protein structure
  • Bovine GH
  • Dot-blot-SNP technique
  • GH gene polymorphism
  • Japanese Black cattle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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